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. 2001 Apr 15;29(8):1801–1807. doi: 10.1093/nar/29.8.1801

Figure 4.

Figure 4

Involvement of individual components in TFO AG30 triplex-mediated DNA repair synthesis of pUSAG15. Individual components were immunodepleted from HeLa nuclear extracts using antibodies against specific components. (A) Visualization of plasmid DNA by ethidium bromide staining. (B) Autoradiogram of the same gel showing labeled nucleotide incorporation indicative of DNA repair synthesis. (C) Quantification of incorporated [α-32P]dCTP in plasmid DNA. Lane 1, CT30 + HeLa nuclear extract; lane 2, AG30 + HeLa nuclear extract; lane 3, AG30 + XPA-depleted HeLa nuclear extract; lane 4, AG30 + TFIID-depleted HeLa nuclear extract; lane 5, AG30 + TFIIH-depleted HeLa nuclear extract; lane 6, AG30 + Pol II-depleted HeLa nuclear extract; lane 7, AG30 + rNTP-lacking HeLa nuclear extract. The data are means obtained from four individual experiments.