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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Immunol. 2011 Jun 3;187(1):133–140. doi: 10.4049/jimmunol.1002742

FIGURE 2. Early IFN-γ production by memory CD4 T cells is not associated with upregulation of T-bet expression or promoter engagement.

FIGURE 2

(A) T-bet expression in resting and antigen-stimulated naive and memory CD4 T cells. OVA-specific naive and memory CD4 T cells were stimulated with OVA/APC as in (A) and T-bet expression assessed by intracellular staining with T-bet specific antibodies. Histograms are gated on CD4+KJ1-26+ cells. (B) T-bet expression in resting and TCR-stimulated polyclonal naive and memory CD4 T cells. Polyclonal naive (CD44lo) and memory (CD44hi) CD4 T cells were sorted from BALB/c splenic CD4 T cells using anti-CD44PE- and PE-coupled magnetic microbeads and MACS™ columns (Miltenyi Biotech, Auburn, CA). Naive (CD44lo) and memory (CD44hi) CD4 T cells were activated with anti-CD3/anti-CD28 antibodies and T-bet expression was assessed at the indicated times. Histograms shown are gated on live CD4+ T cells. (C) T-bet expression as a function of IFN-γ production in resting and antigen-activated OVA-specific naive and memory CD4 T cells. T-bet and IFN-γ expression are shown gated on CD4+KJ1-26+ cells. Results are representative of five independent experiments.