Figure 8.

Expression of LjNTR genes in plant organs. A, Relative LjNTR mRNA levels in roots (R), nodules (N), and leaves (L). The mRNA levels were normalized with respect to ubiquitin, and the LjNTRA mRNA level in roots was arbitrarily assigned a value of 1. All data are means ± se of four biological replicates from two independent treatments. B, Immunoblots of LjNTR proteins in roots, nodules, and leaves. C, Immunoblots of NTRA/B proteins in nodules of L. japonicus (Lj), P. sativum (Ps), P. vulgaris (Pv), and V. unguiculata (Vu). Gels were loaded with 10 μg (B) or 50 μg (C) of protein per lane, and the apparent molecular mass (kD) of the proteins is indicated on the right. Blots are representative of at least two independent protein extractions.