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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Aging Cell. 2010 Aug 15;9(5):776–784. doi: 10.1111/j.1474-9726.2010.00606.x

Fig. 2.

Fig. 2

Immunostaining reveals aldose reductase (AR) expression in aortic endothelial cells and smooth muscle cells of aged animals. Formalin-embedded sections of aortas from young and aged rats were probed with anti-AR IgG, anti-alpha smooth muscle actin (SMA) (a) and anti CD31 (b) IgG, respectively, and visualized after using appropriate second antibody and fluorescent conjugated IgG. Images were visualized under 40× magnification. Immunostaining localized AR to the aortic endothelial and smooth muscle cells in aged animals. Aorta sections stained with appropriate nonimmune serum are presented as negative control.