Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Exp Cell Res. 2011 May 27;317(13):1796–1803. doi: 10.1016/j.yexcr.2011.05.018

Figure 3.

Figure 3

Effects of SB-216763 on adipocyte differentiation and expression of WNT4 and WNT11 in hMSCs. (A) Expression of adipocyte signature genes in hMSCs was inhibited by SB-216763 after transfer to adipocytogenic medium. Agarose gel electrophoretogram shows PCR products for hMSCs (36-year-old man) after indicated days in adipocytogenic medium in the absence or presence of 5 μM SB-216763 (B) Expression of WNT4, WNT11 and adipocyte signature genes was inhibited by 5 μM SB-216763 in hMSCs from 6 subjects, evaluated 7 days after transfer to adipocytogenic medium. There was little or no effect on WNT5A. Each pair of lanes indicates the age in years and the gender (M, male; F, female) of the subject from whom the cells were obtained. (C) Expression of adipocyte signature genes was significantly inhibited by 5 μM SB-216763. Data are shown for 6 samples as the mean and standard error (SE) for each gene (* p<0.05, paired t-test). (D) Expression of WNT4 relative to GAPDH is shown (left panel) for each specimen in DMSO vehicle control and 5 μM SB-216763, and (right panel) as the group means and standard error (*p=0.031, Wilcoxon matched-pairs signed-ranks test). (E) Expression of WNT11 relative to GAPDH is shown (left panel) for each specimen in DMSO vehicle control and 5 μM SB-216763, and (right panel) as the group means and standard error (*p=0.031, Wilcoxon matched-pairs signed-ranks test).