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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 Feb 1;43(5):751–759. doi: 10.1016/j.biocel.2011.01.018

Fig. 3.

Fig. 3

Cell phenotype of HCECs grown in CnT-20 or SHEM medium. A. Representative images of Immunofluorescent staining of stem cell associated markers, p63, Integrin β1 (Itg β1) and EGFR, as well as differentiation markers, Connexin (Cx) 43 and K3 in primary HCECs cultured in CnT-20 or SHEM; Color code: Red is PI; Green is p63, Itg β1, EGFR, Cx43 or K3; and Yellow is green p63 + red PI. B. Percentages of positive cells stained for each marker in primary cultures grown in CnT-20 and SHEM; C. Quantitative real-time PCR data showing the expression levels (relative fold of mRNA) of these markers by HCECs in CnT-20 or SHEM. Data shown as mean ± standard deviation, n=3; *p <0.05, **p < 0.01, ***p < 0.001.