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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 Feb 1;43(5):751–759. doi: 10.1016/j.biocel.2011.01.018

Fig.5.

Fig.5

Effects of Tcf4 silencing by siRNA interference in HCECs cultured in CnT-20. A. Real-time PCR analysis showing the effects of siRNA-Tcf4 (10-50nM) transfection on mRNA expression of β-catenin, Tcf4 and survivin with untreated cells and transfected with non-coding sequence siRNA-F as controls, n=5; B. Western blot results showing the protein levels of β-catenin, Tcf4 and survivin after transfection with siRNA-Tcf4 or –F; C. WST-1 assay showing the cell proliferation indexes after siRNA-Tcf4 transfection. Data are shown the mean and standard deviation of results, ns: no significance, *p <0.05, **p <0.01, ***p <0.001, n=3, compared with siRNA-F transfection.