Skip to main content
. 2001 Apr 15;29(8):1808–1814. doi: 10.1093/nar/29.8.1808

Figure 1.

Figure 1

Figure 1

Structure of the 5′-upstream region of the D-raf promoter and base substitution mutants in potential E2F sites. (A) The 32P-labeled primer complementary to the 5′-region of D-raf cDNA was hybridized with total RNA isolated from 16–20 h embryos and third instar larvae. To align the extended products with the genomic sequence, a parallel dideoxy sequencing reaction was carried out using the same primer (lanes A, C, G and T). The major transcription initiation site is defined as nucleotide position +1. (B) Four closed boxes indicate the E2F recognition sites. The locations of each site relative to the transcription initiation site are indicated by numbering.