Figure 3.

Complex formation between OEBS oligonucleotides and Kc cell nuclear extracts and competition by Draf-E2Fsite3 oligonucleotide. (A) ³²P-labeled OEBS oligonucleotide was incubated with Kc cell nuclear extracts in the presence or absence of anti-dE2F antibody or control normal rabbit antiserum. (B) ³²P-labeled OEBS oligonucleotides were incubated with Kc cell nuclear extracts in the presence or absence of the indicated amounts of competitor oligonucleotides (indicated at the top of each lane). OEBS, oligonucleotide containing the wild-type OEBS; Draf-E2Fsite3wt, oligonucleotide containing E2F site 3 in the D-raf gene promoter; Draf-E2Fsite3mut, oligonucleotide containing base substitutions in E2F site 3 in the D-raf gene promoter.