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. Author manuscript; available in PMC: 2011 Jul 7.
Published in final edited form as: Exp Cell Res. 2009 Sep 2;315(19):3242–3249. doi: 10.1016/j.yexcr.2009.08.020

Fig. 7.

Fig. 7

Colchicine inhibits TNF-α-induced NF-κB activity but not activity induced by overexpression of p65 or c.a. IKKβ in C2C12 cells. Cells were transfected with reporter alone or with plasmids encoding p65 or c.a.IKKβ. (A) Colchicine (100 µM) inhibition of 4 h of TNF-α-induced NF-κB activity (P<0.05). (B) Overexpression of p65 for 2 days strongly induced NF-κB reporter activity and TNF-α addition for 4 h did not further activate the reporter. Colchicine did not inhibit the elevated NF-κB activity due to p65 overexpression in the presence or absence of TNF-α. (C) Overexpression of c.a. IKKβ for 2 days induced NF-κB reporter activity and TNF addition for 4 h did not further activate the reporter. Colchicine did not inhibit the elevated NF-κB activity due to c.a. IKKβ overexpression in the presence or absence of TNF-α.