Figure 5.

Effects of midazolam on IL-1β-induced IκB, p38 MAP kinase or SAPK/JNK phosphorylation. Cultured cells were pretreated with 10 μM midazolam for 60 min, and then stimulated with 10 ng/ml IL-1β or vehicle for 20 min (A) or 30 min (B) in the presence of midazolam. Cell extracts were analyzed by Western blotting using antibodies against phospho-specific IκB, IκB, GAPDH, phospho-specific p38 MAP kinase, p38 MAP kinase, phospho-specific SAPK/JNK or SAPK/JNK. Similar results were obtained with two additional and different cell preparations.