Table 2.
Impact of pretreatment with latrunculin A on the lateral mobility of flotillin-2 cotransfected with flotillin-1 in freshly isolated T-lymphocytes
| Transfection | Pretreatment | MF - SDF-1 (n) | MF + SDF-1 (n) |
|---|---|---|---|
| flo2-EGFP/flo-1-mCherry | No pretreatment | 0.25 ± 0.03 (11) | 0.11 ± 0.04 (12) |
| flo2-EGFP/flo1-mCherry | Preincubation for 30 min with latrunculin A (1 μM) | 0.46 ± 0.07 (12) | 0.66 ± 0.07 (11) |
FRAP analysis involving local bleaching of EGFP was carried out as described in the legend of Figure 6 with cells preincubated in the absence or presence of SDF-1, and without or with pretreatment with latrunculin A as indicated. The mobile fraction (MF) was calculated for each individual cell and then averaged from a total of n = 11 - 12 cells observed in 3 independent experiments, as indicated (mean ± s.e.m.).