Table 2.
Copy number of chloroplast (Cp) and mitochondrial (Mt) genomes in DNA isolated with the CTAB protocol (gDNA) or from isolated nuclei (nDNA)
| Plant | Nuclear genome size (Mb) | DNA isolation protocol | Organelle | gDNA (count) | nDNA (count) | gDNA (%) | nDNA (%) |
|---|---|---|---|---|---|---|---|
| Arabidopsis thaliana | 150 | A | Cp | 317 | 192 | 15 | 9.1 |
| Mt | 3 | 6 | |||||
| Brachypodium distachyon | 300 | B | Cp | 126 | 107 | 2.8 | 2.4 |
| Mt | NA | NA | NA | NA | |||
| Genlisea aurea | 65 | A | Cp | 5467 | 220 | ||
| Mt | 93 | 7 | |||||
| Lemna gibba | 447 | B | Cp | 298 | 73 | 5.5 | 1.3 |
| Mt | NA | NA | NA | NA | |||
| Sorghum bicolor | 692 | B | Cp | 1043 | 578 | 5.3 | 2.9 |
| Mt | 52 | 43 | |||||
| Spirodela polyrhiza | 120 | B | Cp | 778 | 72 | ||
| Mt | NA | NA | NA | NA | |||
| Vaccinium macrocarpon | 450 | C | Cp | 3568 | 695 | ||
| Mt | 785 | 35 | |||||
| Zea mays | 2300 | B | Cp | 278 | 153 | 0.4 | 0.2 |
| Mt | 16 | 6 |
DNA was isolated from Arabidopsis thaliana, Brachypodium distachyon, Genlisea aurea, Lemna gibba, Sorghum bicolor, Spirodela polyrhiza, Vaccinium macrocarpon and Zea mays. Chloroplast DNA contamination in DNA isolated from nuclei or with CTAB. % cpDNA is calculated by ([{# cpDNA copies/#copies of genomic gene}* cp genome size]/1C genome size)*100. Numbers assume a chloroplast and mitochondria genome size of 150 and 500 kb respectively. Data not available (NA).