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Cytoplasmic and nuclear LIMK1 enhances invasion. Invasion assays of MDA-MB-231 cells expressing GFP-only (solid), NLS-GFP-LIMK1 (vertical), NES-GFP-LIMK1 (striped) or GFP-LIMK1 (lines) after 24 hours of invasion through Matrigel-coated invasion chambers. Error bars represent SEM from 8 separate experiments performed in triplicate. Means of each treatment groups differ significantly (p-value less than 0.05) from GFP control (*).
