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. 2011 Jul 7;7(7):e1002117. doi: 10.1371/journal.ppat.1002117

Table 1. Bacterial strains and plasmids.

Strains, Plasmids Description Source and reference
Bacterial strains
E. coli K-12
DH101beta F− endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Invitrogen
φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA
Δ(mrr-hsdRMS-mcrBC) λ−
S17-1 λpir Tpr Smr recA, thi, pro, hsdR − M + RP4:2-Tc:Mu:Km Tn7 [73]
λpir
BL21 CodonPlus F− ompT hsdS(rB −mB −) dcm+ Tetr galλ (DE3) endA Stratagene
(DE3)-RIL Hte [argU ileY leuW Camr] (DE3)- RIL
BL21 λDE3 F− ompT gal dcm lon hsdSB(rB − mB −) gal λDE3 [74]
KB4 BL21 λDE3 stpA hns hha Katja Böhme
Y. enterocolitica
YeO3 6471/76 serotype O:3, patient isolate, wild-type [75]
YeO3-OC 6471/76, Δ(wzx-wbcQ), outer core negative [42]
derivative of 6471/76
YeO3-OCR spontaneous rough derivative of YeO3-OC [42]
YeO3-R2 spontaneous rough derivative of YeO3 [30]
Ye 8081v bioserotype 1A/O:8, patient isolate, wild-type [15]
4620 bioserotype 3/O:9, patient isolate, wild-type A. Fruth
3056 bioserotype 3/O:5,27, patient isolate, wild-type A. Fruth
Y11 bioserotype 4/O:3, patient isolate, wild-type A. Rakin
Y1 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
Y2 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
Y3 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
Y4 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
Y5 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
Y8 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
Y32 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
Y33 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
Y34 bioserotype 4/O:3, patient isolate, wild-type E. Strauch
YE01 Y11, ΔrovA, CmR This study
YE12 Y1, ΔrovA, CmR This study
YE13 YE12, ProvAO:3::rovA S98, CmR KnR This study
YE14 YE12, ProvAO:3::rovA P98, CmR KnR This study
YE15 Y1, PinvAΔIS1667 This study
YE18 Y1, ΔyadA, TetR This study
YE21 Y1, ΔinvA, KnR This study
Plasmids
pASKIBA43+ overexpression vector, ApR IBA
pBAD33 overexpression vector, CmR [76]
pBADmycA-HisA overexpression vector, ApR Invitrogen
pET28a overexpression vector, KnR Novagen
pFU32 ColE1, promoterless luc, TetR lab collection
pFU49 pBAD33, invA + O:3, CmR This study
pFU99 pSC101*, promoterless lacZ, CmR lab collection
pFU100 R6K, mobRP4, promoterless luxCDABE, CmR lab collection
pFU102 pFU100, rovA::CmR This study
pFU109 pSC101*, promoterless lacZ, KnR lab collection
pFU114 pFU100, ‘invA’ (nt 40–339), CmR This study
pFU119 pFU109, ProvAO:3::rovA O:3, KnR This study
pFU129 pFU109, ProvAO:8::lacZ, KnR This study
pFU130 pFU109, ProvAO:3::lacZ, KnR This study
pFU138 pFU119, ProvAO:3::rovA O:8, KnR This study
pFU156 pET28a, rovA+ O:8, KnR This study
pFU157 pET28a, rovA+ O:3, KnR This study
pFU167 pFU102, rovA::CmR, sacB This study
pFU170 pSC101*, Pinv O:8::luxCDABE, KnR This study
pFU171 pSC101*, Pinv O:3::luxCDABE, KnR This study
pFU172 pSC101*, Pinv O:3ΔIS::luxCDABE, KnR This study
pFU175 pSC101*, promoterless luxCDABE, KnR lab collection
pFU182 pBAD33, invA + O:8, CmR This study
pFU184 pFU119, ProvAO:3::rovA O:3, KnR This study
pFU185 pFU138, ProvAO:3::rovA S98P, KnR This study
pFU187 pFU167, yadA::TetR This study
pFU188 pBADmycA-HisA, yadA + O:3, ApR This study
pFU190 pFU167 PinvAO:3 (−1887 to −1627/−249 to −1), TetR This study
pFU194 pFU175, −1169 bp invA upstream region, KnR This study
pFU195 pFU175, −448 bp invA upstream region, KnR This study
pFU196 pFU175, −248 bp invA upstream region, KnR This study
pFU197 pFU175, −146 bp invA upstream region, KnR This study
pFU198 pFU175, −72 bp invA upstream region, KnR This study
pFU199 pASKIBA43+, hns+ O:3, ApR This study
pFU201 pSC101*, Pinv O:3(−248 to −146)-luxCDABE, KnR This study
pFU202 pSC101*, Pinv O:3(−448 to −146)-luxCDABE, KnR This study
pFU213 pFU114, ‘invA’ (nt 40–339), KnR This study
pFU220 pBAD33, yadA + O:3, CmR This study
pJE4 R6K, sacB+, KnR J. Eitel
pZA31luc expression vector, p15A, PLtetO-1, CmR [77]
pZS*24MCS expression vector, pSC101*, Plac/ara-1, KnR [77]