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. 2011 Jul 7;6(7):e21806. doi: 10.1371/journal.pone.0021806

Figure 1. N-terminal PRL fragments content in the anterior pituitary varies along the estrous cycle.

Figure 1

A: Recombinant 23 kDa PRL (r23-PRL), recombinant 16 kDa prolactin (r16-PRL) or pituitary protein extracts treated with β-mercaptoethanol (P+β, reducing conditions) or without β-mercaptoethanol (P, non-reducing conditions) were incubated with anti-recombinant rat PRL antibody (anti rrPRL, 1∶25000). The antibody recognizes both PRL forms. B: Anterior pituitaries from rats euthanized at diestrus I or proestrus were processed for western blot. Upper panel: Each column represents the mean ± SE of the relative increment of N-terminal PRL fragments content with respect to diestrus I. Data of each column were normalized to β-actin expression (n = 4–7 animals per group). *p<0.01 vs. diestrus I, Student's t test. Lower panel: Representative blot of pituitary proteins from rats euthanized at diestrus I or proestrus.