Domains of hMLH1 interacting
with hMLH3, hPMS1 and hPMS2. The β-gal
activities in yeast cells containing various lexA DNA BD and VP16
transcriptional AD plasmids. The left figure represents schematic
diagrams of the full-length (residues 1–756) and the 15 hMLH1 deletion constructs in AD plasmids. Lane
hMLH3, hPMS1 or hPMS2 indicates the β-gal
activities of yeast cells that contain the full-length hMLH3, hPMS1 or hPMS2 BD plasmid and
the corresponding truncated hMLH1 AD plasmid. The
mean and SD of three independent transformants are shown. The mean β-gal activities in yeast cells containing
only hMLH3, hPMS1 or hPMS2 BD plasmid are 0.3, 0.1 or 0.1 U, respectively.