Figure 2.

Summary of the two-hybrid assay, GST-IVTT and immunoprecipitation study between the full-length hMLH1 and various hPMS2 deletion constructs. These three assays were performed as described in Materials and Methods. The left figure represents schematic diagrams of the full-length (residues 1–862) and the twenty-six hPMS2 deletion constructs. The mean and SD of β-gal activities in three independent yeast transformants containing various hPMS2 deletion constructs in the BD plasmid and the full-length hMLH1 cDNA in the AD plasmid are indicated in the two-hybrid lane. The values in parentheses represent the mean β-gal activity in the yeast cell containing only the corresponding BD plasmid. Results of the GST-IVTT assay are presented as the mean and SD of three separate experiments and are indicated as relative values when the binding ability of the full-length hPMS2 to GST-hMLH1 is 100. Lane IP indicates the presence (+) or absence (–) of the immunoprecipitated band. ND, not detected.