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Competitive inhibition of hPMS2 binding by hPMS1 or hMLH3 when complexed with GST-hMLH1. The GST-hMLH1 and 2 µl of 35S-labeled IVTT-hPMS2 were incubated with indicated amounts of unlabeled IVTT-luciferase, IVTT-hPMS1 or IVTT-hMLH3. Then hPMS2 complexed with GST-hMLH1 was resolved by 10–20% SDS–PAGE and examined by BAS-1500. The arrow indicates the position of hPMS2 protein. Both hPMS1 and hMLH3 compete with hPMS2 for the binding to hMLH1.
