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. 2011 Jul 7;6(7):e22023. doi: 10.1371/journal.pone.0022023

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Delgado et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Quantitative RT-PCR analysis of several genes within pathways critical for progenitor cell maintenance in HBEC3 KT cells five days after varying culture conditions. Transition of HBEC3 KT cells from standard growth media to differentiation media while cultured in 2D results in the significant upregulation of many genes within progenitor-associated pathways (Columns 1 and 2) relative to HBEC3 KT cells cultured within Matrigel™ with differentiation media and IMR90 fibroblast growth stimulus (Columns 3 and 4) (P = 0.00185). Results are from two independent experiments (Columns 1 and 3 and 2 and 4, respectively). Experimental samples normalized to gene expression in HBEC3 KT cells coincidentally maintained in growth media.