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Modification-dependent endonuclease activity of MspJI and homologs. DNA was incubated at 37 °C with 1 unit of each of the indicated homolog, a cocktail of the homologs (0.2 unit each), or BstNI (CC↓WGG). Control DNA was incubated in parallel without enzyme addition. (A) pBR322 dcm+ (CmCWGG methylated) plasmid DNA. (B) A 3-kb PCR amplified DNA fragment (unmodified) DNA. (C). T4gt (hydroxymethylated) genomic DNA. (D) T4wt (glucosylated) genomic DNA.
