Fig. 5.

GSK-3β–dependent Snail degradation occurs as a result of humoral factor signals that drive liver regeneration. (A) Western blot analyses of Snail, PCNA, and GSK-3β in age-matched normal mouse liver (no injection) and in the liver of adult mice at 1 d after injection of HGF, IL-6, and cholic acid. (B) Western blot analyses of total cell lysates derived from the liver after PH or hydrodynamic injection of Snail siRNA into adult mice. P, phosphorylated. (C) Western blot analyses of Snail and PCNA in the liver of wild-type, FXR^−/−, and IL-6^−/− mice at 2 d after hydrodynamic injection of a control siRNA or Snail siRNA into adult mice. (D) Oil red O staining of the liver after PH or hydrodynamic injection of Snail siRNA into adult mice to detect lipid droplet formation and accumulation in hepatocytes. (Scale bar, 50 μm.)