Fig. 1.

OTLD1 is an otubain-like protein that interacts with KDM1C. (A) Sequence alignment and domain structure of A. thaliana OTLD1 and its homolog from rice (O. sativa). Conserved OTU and UBA domains are indicated by shaded and boxed residues, respectively. (B) Specific interaction of OTLD1 with KDM1C in the two-hybrid system. (Left) Growth in the absence of histidine, tryptophan, and leucine. (Right) Growth in the absence of tryptophan and leucine. Growth in histidine-deficient medium represents selective conditions for protein–protein interactions.