Fig. 6.

ZLD potentiates adaptive immunity as an adjuvant. WT mice (n = 8–9 per group) were immunized twice, 1 wk apart, subcutaneously with vehicle (Veh), 30 μg OVA, or OVA plus 10 μg ZLD (OVA+ZLD). (A) Total serum anti-OVA IgG 6 wk after the initial vaccination was measured by ELISA. (B) Antigen-specific IFN-γ release by splenocytes. The splenocytes were restimulated with OVA (100 μg/mL) in vitro for 3 d. Data shown are pooled from two independent experiments. *P < 0.05 by one-way ANOVA with Dunnett's post hoc test.