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. 2011 Jul 7;6(7):e21939. doi: 10.1371/journal.pone.0021939

Figure 3. Analysis of the binding of the GR AF1 to TBP by SPR.

Figure 3

TBP was immobilized to the Fc2 channel of C1 chip as the ligand, the Fc1 was equally treated but without TBP as the control channel. AF1 was used as the analyte to measure the binding as described in “Materials and methods”. A multi-cycle kinetics assay was run by Biacore X-100 plus. A) Raw sensorgrams of Fc1 and Fc2 to show the specific binding of AF1 to TBP. B) The adjusted sensorgrams (Fc2-Fc1) were overlaid and fitted for kinetics with 1∶1 binding model. The measured sensorgrams are shown in color, and the fitted ones in black. The experiments were repeated twice.