Accumulation of incised photolesions
in spermatocytes, round spermatids and MNC. (A–C) Cells incubated with (filled columns) or without
(open columns) HU (2 mM) and AraC (0.1 mM) for 1 h at 32°C
(SC and RST) or 37°C (MNC), followed
by analysis with conventional SCGE. (D–F) Analysis with SCGE in combination with (filled
columns) or without (open columns) T4Endo V. In (A), (B), (D) and
(E), comets were sorted according to their DNA content. Means ± SD of four experiments (A), (B), (D)
and (E) or data from one representative experiment (C) and (F) are
shown.