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. 2011 Jul 7;6(7):e22086. doi: 10.1371/journal.pone.0022086

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Grainger, Putnam.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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60x confocal microscopy images of days 5 (A) and 10 (B) capillaries after 70 kDa dextran tracer addition (red), fixation with 10% formalin, and human CD31 staining (green) of HUVECs. Inset images: HUVECs (top) and Dextran (bottom) show hollow lumens with or without dextran. A) Red tracer is present within the lumens of the capillaries, demonstrating a low resistance to permeability. B) Red tracer is excluded from the lumens of the capillaries, demonstrating an increased resistance to permeability as the capillaries mature over time.