Table IV.
Frequency of sequence characteristics among single cells
TCRα |
*Incidence of predicted V-J non-germline junctional sequence size |
#TRAV16 (#TRAV6) |
Total # unique amino acid sequences |
||||
---|---|---|---|---|---|---|---|
0 aa | 1 aa | 2 aa | 3 aa | 4 aa | |||
Mouse 1 | 3 | 9 | 11 | 2 | 0 | 13 (4) | 25 |
Mouse 2 | 2 | 9 | 4 | 1 | 0 | 11 (3) | 16 |
Mouse 3 | 4 | 6 | 7 | 3 | 0 | 11 (1) | 20 |
TCRβ |
*Incidence of maximal predicted V-D or D-J non- germline junctional sequence size |
# sequences with amino acids RG in TRBD position |
Total # unique amino acid sequences |
||||
0 aa | 1 aa | 2 aa | 3 aa | 4 aa | |||
Mouse 1 | 2 | 8 | 8 | 0 | 0 | 16 | 18 |
Mouse 2 | 3 | 9 | 4 | 0 | 0 | 14 | 16 |
Mouse 3 | 0 | 3 | 5 | 0 | 1 | 8 | 9 |
A composite of data is shown for three individual mice. The number of sequences with various sizes of predicted non-germline amino acids between V-J α, V-Dβ, or D-Jβ is shown. Also shown are the numbers of sequences with TRAV16 or TRAV6 usage, or with the amino acids RG in the position of TRBD.