Table VI.
Shared junctional sequences in the d-NALT, lung and BAL in Mouse 2 and Mouse 3
| d-NALT V alpha | Lung V Alpha | BAL V Alpha |
|---|---|---|
| A. Mouse 2: SHARED SEQUENCES BETWEEN URT AND LRT (number of single cells per tissue sharing sequence) | ||
| REDNYAQGLTFGLGTR (2) | REDNYAQGLTFGLGTR (3) | REDNYAQGLTFGLGTR (3) |
| REDNTGNYKYVFGAGT (2) | REDNTGNYKYVFGAGT (1) | REDNTGNYKYVFGAGT (1) |
| REGDGTGSKLSFGKGA (2) | REGDGTGSKLSFGKGA (1) | REGDGTGSKLSFGKGA (3) |
| GDPDNAGAKLTFGGGT (1) | GDPDNAGAKLTFGGGT (1) | GDPDNAGAKLTFGGGT (2) |
| GGSNTNKVVFGTGTRL (3) | GGSNTNKVVFGTGTRL (3) | |
| RDNNYAQGLTFGLGTR (3) | RDNNYAQGLTFGLGTR (2) | |
| REEYANKMIFGLGTIL (2) | REEYANKMIFGLGTIL (1) | |
| MRDTNAYKVIFGKGTHL (1) | MRDTNAYKVIFGKGTHL (4) | |
| REDNNNNAPRFGAGTK (1) | REDNNNNAPRFGAGTK (2) | |
| DPMDSNYQLIWGSGTK (1) | DPMDSNYQLIWGSGTK (1) | |
| REVDTNTGKLTFGDGT (1) | REVDTNTGKLTFGDGT (1) | |
| SIGTGNTGKLIFGLGT (1) | SIGTGNTGKLIFGLGT (1) | |
| B. MOUSE 3: SHARED SEQUENCES BETWEEN URT AND LRT (number of single cells per tissue sharing sequence) | ||
| REIMDSNYQLIWGSGT (8) | REIMDSNYQLIWGSGT (3) | REIMDSNYQLIWGSGT (4) |
| REGDSNNRIFFGDGTQ (1) | REGDSNNRIFFGDGTQ (1) | |
| TDYSNNRLTLGKGTQVV (1) | TDYSNNRLTLGKGTQVV (1) | |
| AGDTNAYKVIFGKGTHL (2) | AGDTNAYKVIFGKGTHL (1) | |
Legend: TCRα sequences from d-NALT, BAL and lung are listed to demonstrate sharing of precise TCRα junctions. In mouse 2, there were 87 clones for which TCR α sequences could be defined. Listed are the 50 sequences that were shared between URT and LRT samples. In mouse 3, there were 59 clones for which TCR α sequences could be defined. Listed are the 22 sequences that were shared between URT and LRT.