Fig 3.

CT-induced I_SC is chloride dependent. (A) CT (10 nM) was added from the basolateral side in regular Cl⁻ containing Krebs-Henseleit medium and Cl⁻ free Krebs-Henseleit medium where Cl⁻ was replaced with equimolar gluconate. Data shown here are 1 and 5 min. after adding CT, calculated as percentage of Cl⁻ containing Krebs-Henseleit medium. For studying the effects of different inhibitors, CT (10 nM) was added from the basolateral side after establishment of the baseline. Ten minutes later, inhibitors: (B) control = vehicle, (C) Bumetanide (100 μM), (D) barium chloride (5 mM) were added from the basolateral side, and (E) CFTR_inh-172 (10 μM) was added from the apical compartment of the T84 monolayer. One representative tracing of each is shown here. (F) Data shown here are the values of I_SC 10 min. after addition of different inhibitors (control = vehicle, Bumetanide, barium chloride, CFTR_inh-172) (arrowhead in B, C, D and E), calculated as percentage of control. Results represent mean ± S.E.M. of three or more independent experiments in all above figures. **P < 0.001.