Fig 6.

CT increased CFTR level on apical membrane. T84 cells were plated in transwells. Cells were treated with CT (10 nM) or vehicle for 20 min. and then subjected to biotinylation at 4°C utilizing sulfo-NH-SS-biotin. Biotinylated proteins were extracted with streptavidin-agarose, and surface and total fractions were run on 8% SDS-PAGE. The blot was immunostained with rabbit anti-CFTR antibody. Representative blots of three separate experiments are shown. Quantification data from densitometric analysis are shown from three different experiments. Values are expressed as percentage of control and represent mean ± S.E. *P < 0.05.