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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Clin Cancer Res. 2011 May 12;17(13):4309–4319. doi: 10.1158/1078-0432.CCR-10-1820

Figure 4. Silencing of MAGE-A induces p53-dependent Bax/Bak expression and repression of survivin.

Figure 4

MM.1r cells were transduced with MAGE-A-targeted (shMA) or p53-targeted (shp53) shRNA lentiviral constructs, both targeted constructs, or controls as indicated and cells were harvested at 48 hrs. A. Bax, Bak, and survivin mRNA expression was assessed by qRT-PCR. B. Protein expression of Bcl-2 proteins and survivin was assessed by western blot. C. p53 mRNA expression was assessed by qRT-PCR and protein expression by western blot. D. Cells were incubated with 40 nM MG132 prior to harvest and lysates were immunoprecipitated (IP) with p53-specific mAb or isotype control mu IgG (iso). Ubiquitinylation of p53 was assessed by western blot.