Fig. 2.
A, immunocytochemical analysis for (A) E-cadherin and (B) vimentin protein expression in MDA-MB-231 cells after 24-hour treatment with DMSO (control) or 2.5 μmol/L BITC. C, E-cadherin mRNA expression in MDA-MB-231 and MCF-7 cells after 8- or 16-hour treatment with DMSO (control) or BITC (1 or 2.5 μmol/L). D, E-cadherin luciferase activity in MDA-MB-231 cells treated for 8 or 16 hours with DMSO (control) or BITC (1 or 2.5 μmol/L). Columns, mean (n=3); bars, SD. *Significantly different (P<0.05) compared with DMSO-treated control by Student’s t-test. Each experiment was repeated at least twice. Representative data from one such experiment are shown.
