Table 3.

Frequency of methylation

A. Fifteen genes in gene evaluation set
Number of methylation positive/number of total cases (Methylation positive %)
Markers	Serum or plasma (Cancer)	Serum (Control)	Cutoff values
APC	9/25(36%)	2/30(7%)	0
AIM1	4/17(24%)	1/15(7%)	0
CyclinD2	4/17(24%)	3/35(9%)	0
CALCA	12/17(71%)	23/35(66%)	0
CDH1	10/17(59%)	1/15(7%)	0.3
DCC	3/17(18%)	0/136(0%)	0
p16	0/25(0%)	0/30(0%)	0
MGMT	5/25(20%)	1/30(3%)	0
Rassf1A	2/25(8%)	1/30(3%)	0.1
MINT31	0/20(0%)	0/30(0%)	0
CyclinA1	2/20(10%)	0/155(0%)	0
ESR	1/20(5%)	0/35(0%)	0
HIC1	10/20(50%)	42/614(7%)	0
PGP 9.5	3/20(15%)	9/318(3%)	0
TIMP3	5/20(25%)	0/30(0%)	1

B. Six genes in gene independent set
Number of methylation positive/number of total cases (Methylation positive %)
Markers	Serum or plasma (Cancer)	Serum (Control)	P	Sensitivity (%)	Specificity (%)
APC	12/76(16%)	3/30(10%)	0.55	15.8	90
AIM1	14/76(18%)	1/30(3%)	0.06	18.4	96.7
CDH1	47/76(62%)	9/30(30%)	0.003	61.8	70
DCC	27/76(36%)	0/30(0%)	0.0002	35.5	100
MGMT	13/76(17%)	1/30(3%)	0.11	17.1	96.7
Rassf1A	6/76(8%)	1/30(3%)	0.67	7.9	96.7
1 of 6 markers	64/76(84%)	13/30(43%)	<.0001	84.2	56.7