The level of proinflammatory mediators, such as MCP-1, KC and IP-10 are measured in lung homogenates obtained from air- or CS-exposed WT and FoxO3-/- mice for 3 days (A), 8 weeks (B) and 4 months (C). Data are shown as mean ± SEM (n= 3 to 4 per group). *P < 0.05, **P < 0.01, ***P < 0.001, significant compared to corresponding air-exposed mice. +P < 0.05, ++P < 0.01, +++P < 0.001 significant compared with CS-exposed WT mice.