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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Apr 4;35(8):1509–1518. doi: 10.1111/j.1530-0277.2011.01487.x

Figure 1. Bone marrow (BM) transplantation sufficiently replaced Kupffer cells with BM-derived cells in the livers of alcohol-fed mice.

Figure 1

(A-C) Bone marrow (BM) transplantation was performed to generate the chimeric mice; (A-B) BM from β-actin promoter-driven GFP Tg mice was transplanted into WT mice treated with liposomal clodronate and whole body irradiation. Liver mononuclear cells were isolated from control and ethanol diet-fed mice. F4/80 positive GFP-expressing hepatic non-parenchymal cells were determined by FACS analysis. (C) Liver sections from control and ethanol diet-fed mice were stained with anti-F4/80 (upper, red color) and desmin (lower, red color) antibodies as the markers for HSCs and Kupffer cells, respectively. Green fluorescence signals represent endogenous GFP expression as BM-derived cells.