. Author manuscript; available in PMC: 2012 Jul 15.

Published in final edited form as: J Immunol. 2011 Jun 15;187(2):1006–1014. doi: 10.4049/jimmunol.1003521

Table II.

Oligoclonality in the T Cell Repertoire of CAS Valves and in Peripheral Blood Phenotypic Subsets, Determined by β-Chain Nucleotide Sequencing and by β-Chain Length Distribution Analysis

ID^†	CAS Score^†	Valve			Peripheral Blood
		Repertoire Complexity		CD8^*	CD8⁺ CD28^null			CD8⁺ CD28⁺			CD4⁺
		Repertoire Complexity		CD8^*	% CD8 T Cells	Repertoire Complexity		% CD8 T Cells	Repertoire Complexity		Repertoire Complexity

		SDI^‡	mHD^‡	%		SDI	mHD		SDI	mHD	SDI	mHD
T55	2	0.27	69.9	99	38	0.57	64	72	0.67	48.2	0.99	20.2
T19	3	0.68	74.6	-	54	-	-	46	-	-	-	-
T10	4	0.84	56.7	61	90	-	-	10	-	-	-	-
T54	5	0.91	55.8	50	81	0.05	62.8	19	0.89	54.6	0.99	20.3
T57	5	0.82	51.4	44	72	0.75	60.9	28	0.79	35.6	0.9	11.2
T56	7	0.98	44.5	15	79	0.52	67.4	21	0.96	49.8	0.99	38.1
B38	1	0.76	68.7	83	60	0.89	50.6	40	0.99	29.8	1	10.9
B14	6	0.93	61.3	70	74	-	-	26	-	-	-	-
B46	7	-	50.5	-	72	0.85	61.1	28	0.78	40	0.94	16.3

Mean	4.4	0.8	59.3		68.9	0.6	61.1	32.2	0.8	43.0	1.0	19.5

CD8 refers to percentage of CD3⁺ T cells in the valve that are CD8⁺ by double immunostaining. (data not shown for 7 additional samples)

^†

ID case number, prefix T or B refers to tricuspid or bicuspid valve. CAS score=Measure of severity of pathologic changes defined in the methods.

^‡

Simpson’s Diversity Index, an index of repertoire diversity determined from β-chain sequencing, 1.0=polyclonal. mHD=Mean Hamming Distance, the average of the distance between the observed TCR β-chain length distribution and a polyclonal reference, 0=polyclonal.