Table II.
ID† | CAS Score† | Valve
|
Peripheral Blood
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Repertoire Complexity | CD8* | CD8+ CD28null |
CD8+ CD28+ |
CD4+ |
||||||||
% CD8 T Cells | Repertoire Complexity | % CD8 T Cells | Repertoire Complexity | Repertoire Complexity | ||||||||
SDI‡ | mHD‡ | % | SDI | mHD | SDI | mHD | SDI | mHD | ||||
T55 | 2 | 0.27 | 69.9 | 99 | 38 | 0.57 | 64 | 72 | 0.67 | 48.2 | 0.99 | 20.2 |
T19 | 3 | 0.68 | 74.6 | - | 54 | - | - | 46 | - | - | - | - |
T10 | 4 | 0.84 | 56.7 | 61 | 90 | - | - | 10 | - | - | - | - |
T54 | 5 | 0.91 | 55.8 | 50 | 81 | 0.05 | 62.8 | 19 | 0.89 | 54.6 | 0.99 | 20.3 |
T57 | 5 | 0.82 | 51.4 | 44 | 72 | 0.75 | 60.9 | 28 | 0.79 | 35.6 | 0.9 | 11.2 |
T56 | 7 | 0.98 | 44.5 | 15 | 79 | 0.52 | 67.4 | 21 | 0.96 | 49.8 | 0.99 | 38.1 |
B38 | 1 | 0.76 | 68.7 | 83 | 60 | 0.89 | 50.6 | 40 | 0.99 | 29.8 | 1 | 10.9 |
B14 | 6 | 0.93 | 61.3 | 70 | 74 | - | - | 26 | - | - | - | - |
B46 | 7 | - | 50.5 | - | 72 | 0.85 | 61.1 | 28 | 0.78 | 40 | 0.94 | 16.3 |
| ||||||||||||
Mean | 4.4 | 0.8 | 59.3 | 68.9 | 0.6 | 61.1 | 32.2 | 0.8 | 43.0 | 1.0 | 19.5 |
CD8 refers to percentage of CD3+ T cells in the valve that are CD8+ by double immunostaining. (data not shown for 7 additional samples)
ID case number, prefix T or B refers to tricuspid or bicuspid valve. CAS score=Measure of severity of pathologic changes defined in the methods.
Simpson’s Diversity Index, an index of repertoire diversity determined from β-chain sequencing, 1.0=polyclonal. mHD=Mean Hamming Distance, the average of the distance between the observed TCR β-chain length distribution and a polyclonal reference, 0=polyclonal.