Table III.
Two Examples of Shared T Cell Clones Identified in the Aortic Valve and in Peripheral Blood Phenotypic Subsets with Identical β-Chain Sequences, Indicating Clonal trafficking
| GenBank Accession number | Case | Sample Source | CDR3 length | CDR3 Region Nucleotide Sequence, Junction Analysis and Translation* | Number sequences in clone† | Clonotype frequency in BV family (%) | Clonotype frequency in repertoire (%) | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JF731133 | T55 | Tricuspid Valve | 12 | TGTGCCAGCAGCTTAGctttGGCtttCAATGAGCAGTTCTTC | 18 | 85.7 | 10.94 | ||||||||||||||||||
| C | A | S | S | L | A | L | A | F | N | E | Q | F | F | ||||||||||||
| JF731134 | T55 | Circulating CD8+CD28neg T cells | 12 | TGTGCCAGCAGCTTAGctttGGCtttCAATGAGCAGTTCTTC | 3 | 17.7 | 1.51 | ||||||||||||||||||
| C | A | S | S | L | A | L | A | F | N | E | Q | F | F | ||||||||||||
| JF731135 | T55 | Circulating CD8+CD28+ T cells | 12 | TGTGCCAGCAGCTTAGctttGGCtttCAATGAGCAGTTCTTC | 4 | 20 | 1.97 | ||||||||||||||||||
| C | A | S | S | L | A | L | A | F | N | E | Q | F | F | ||||||||||||
| JF731138 | T55 | Tricuspid Valve | 17 | TGTGCCAGCAGCTTAaatGACAGGGGagtaggacttagCTCCTACGAGCAGTACTTC | 2 | 9.5 | 1.21 | ||||||||||||||||||
| C | A | S | S | L | N | D | R | G | V | G | L | S | S | Y | E | Q | Y | F | |||||||
| JF731139 | T55 | Circulating CD8+CD28+ T cells | 17 | TGTGCCAGCAGCTTAaatGACAGGGGagtaggacttagCTCCTACGAGCAGTACTTC | 11 | 55 | 5.41 | ||||||||||||||||||
| C | A | S | S | L | N | D | R | G | V | G | L | S | S | Y | E | Q | Y | F | |||||||
*
Upper case denotes germ line residues encoded respectively by the TRBV element, TRBD element (underlined) and TRBJ element. Lower case denotes N or P non-germline encoded nucleotides.
†
The number of identical TCRB sequences found in the sample that comprises the expanded clone, only one sequence of which has been entered in Genbank.