Table 1.
PCR primers for BRAF, KRAS and NRAS hotspot mutations, and RET/PTC1, RET/PTC3 and NTRK1 chromosomal rearrangements
| Gene | Codons | Primer Sequences (5'–3'; a, forward; b, reverse) |
|---|---|---|
| KRAS | 12/13 | a 5'-GGCCTGCTGAAAATGACTGAA-3' |
| b 5'-GGTCCTGCACCAGTAATATGC-3' | ||
| KRAS | 61 | a 5'-CAGGATTCCTACAGGAAGCAAGTAG-3' |
| b 5'-CACAAAGAAAGCCCTCCCCA-3' | ||
| NRAS | 12/13 | a 5'-ATGACTGAGTACAAACTGGT-3' |
| b 5'-CTCTATGGTGGGATCATATT-3' | ||
| NRAS | 61 | a 5'-TCTTACAGAAAACAAGTGGT-3' |
| b 5'-AGCGGATAACAATTTCACACAGGC CAA AAA TTTAATCAGTGGA-3' | ||
| BRAF | 600 | a 5'-TGTAAAACGACGGCCAGTCATAATGCTTGCTCTGA TAG GA-3' |
| b 5'-AGCGGATAACAATTTCACACAGGCCAA AAATTTAATCAGTGGA-3' | ||
| RET/PTC1 | a 5'-GCT GGA GAC CTA CAA ACT GA-3' | |
| b 5'-GTT GCC TTG ACC ACT TTT C-3' | ||
| Nested primer | a 5'-ACA AAC TGA AGT GCA AGG CA-3' | |
| b 5'-GCC TTG ACC ACT ACT TTT CCA AA-3' | ||
| RET/PTC3 | a 5'-AAG CAA ACC TGC CAG TGG-3' | |
| b 5'-CTT TCA GCA TCT TCA CGG-3' | ||
| Nested primer | a 5'-CCT GCC AGT GGT TAT CAA GC-3' | |
| b 5'-GGC CAC CGT GGT GTA CCC TG-3' | ||
| NTRK1 | a 5'-TGAGCAGATTAGACTGATGG-3' | |
| b 5'-GGAAGAGGCAGGCAAAGAC-3' | ||
| Nested primer | a 5'-GCTGCCGAAGAAAAGTACTC-3' | |
| b 5'-TTTCGTCCTTCTTCTCCACC-3' |