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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Cancer Prev Res (Phila). 2011 Apr 4;4(7):1052–1060. doi: 10.1158/1940-6207.CAPR-11-0067

Figure 2. P-S/DFMO decreases proliferation, induces cell death by apoptosis, and reduces polyamine levels in human colon xenografts in mice.

Figure 2

Changes in proliferation and apoptosis indices in response to P-S, DFMO, or combination of both. Percentage of proliferation (A) and apoptotic cells (B) of xenograft tumors were determined as described in Materials and Methods. Left: Images of tissue sections from xenograft tumors treated with either vehicle (control), P-S, DFMO, or both and stained for Ki-67 expression (proliferation marker) or by the TUNEL method (apoptosis) as described in Materials and Methods. Negative controls are shown as insets at the top right corner of each image. Right: The number of Ki-67 and TUNEL-positive cells were counted and expressed as percentage of the total number of cells per field. *Significantly different from control group (p<0.05, one-way ANOVA test; IHC staining, ×20). C. P-S/DFMO reduces the level of polyamines in human colon xenografts in mice. Polyamine levels were measured by HPLC as described in Materials and Methods. Polyamine content in the tissue samples isolated from tumors treated with P-S, DFMO or both. Data are expressed as percentage of control. Values are shown as mean±SEM of 5 independent tumors. *Significantly different from control group (p<0.05, one way ANOVA test).