Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Melanoma Res. 2011 Aug;21(4):285–297. doi: 10.1097/CMR.0b013e32834495c3

Fig. 3.

Fig. 3

Validation of candidate target genes regulated by PEDF in A375 melanoma cells. (a) Quantitative RT-PCR analysis of mRNA levels of genes related to: 1) angiogenesis/adhesion/migration/invasion: COL4A2, FN1, IL8 and TGFA; 2) melanoma progression: FGF13, IGFBP3, JAG1 and LGALS3; 3) melanoma markers: MIA and S100B; 4) melanin secretion: MLPH and RAB27A. Empty bars correspond to A375-pCEP4 Pool cells and filled bars to A375-pCEP4-PEDF Pool cells. For each gene mRNA levels are shown relative to A375-pCEP4 Pool after normalization to GAPDH. Bars represent average ± SD. Statistical significance was determined by Student’s t-test or Welch-corrected t-test (the latter for COL4A2, TGFA, LGALS3, MLPH and S100B) (*, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001). (b) ELISA analysis of secreted IL8 protein levels in 48 h CM from A375-pCEP4 Pool and A375-pCEP4-PEDF Pool cells. Bars represent average ± SD. Statistical significance was determined by Student’s t-test (****, p<0.0001). Results are representative of two independent experiments.