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. Author manuscript; available in PMC: 2012 Jul 13.
Published in final edited form as: J Am Chem Soc. 2011 Jun 14;133(27):10434–10447. doi: 10.1021/ja110196d

Figure 5.

Figure 5

(a) [M + H]+ signal of the 5′-dA isolated out of the SPL reaction with the d3-SP TpT (2) which contains a deuterium at the 6-HproS position as the enzyme substrate, the 252.1 signal suggests that no deuterium was incorporated into the 5′-dA. (b) [M + H]+ signal of the 5′-dA isolated with d4-SP TpT (4) which contains a deuterium at the 6-HproR position as the enzyme substrate. The observed 253.1 signal suggests that a deuterium atom was incorporated into the 5′-dA.