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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Neurosci Res. 2011 Jun 14;89(9):1363–1374. doi: 10.1002/jnr.22675

Figure 2. Smo agonist purmorphamine dose-dependently stimulates the expression of GFP in HB9::GFP embryoid bodies.

Figure 2

EBs were cultured for 5 days with 1 µM retinoic acid and purmorphamine (main graph) or Shh (inset) at indicated concentrations. Images of GFP fluorescence were taken for 6–9 EBs in each treatment group, and fluorescence was quantified using ImageJ. Error bars are SEM.