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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Neurosci Res. 2011 Jun 14;89(9):1363–1374. doi: 10.1002/jnr.22675

Figure 5. PACAP antagonizes the effect of purmorphamine on Gli1 mRNA in neuralized embryoid bodies.

Figure 5

(A) Time course of Gli1 gene expression. EBs were cultured with 1 µM retinoic acid with or without purmorphamine (1 µM) for the indicated number of hours. Results are mean Gli1 mRNA levels (arbitrary units) from 5–6 individual samples ± SEM. (B,C) EBs cultured for 3 (B) or 5 (C) days with 1 µM retinoic acid and purmorphamine (1 µM) and/or PACAP38 (10 nM). Results are mean Gli1 mRNA levels expressed as fold increase over control from 6–18 individual samples ± SEM. n/s: non-significant, *p<0.05, **p<0.01, ***p<0.001.