In the absence of agonist, dynamin tetramers may be bound to eNOS and present in the cytosol. Upon stimulation with agonist, eNOS induces the S-nitrosylation of dynamin that, in turn, promotes the dynamin recruitment to the neck of invaginated pits on the plasma membrane. The S-nitrosylation also increases dynamin self-assembly and its GTPase activity, thereby enhancing rate of vesicle scission from the plasma membrane and internalization of activated receptor.