. Author manuscript; available in PMC: 2012 Jul 15.

Published in final edited form as: J Mol Biol. 2011 May 13;410(3):411–423. doi: 10.1016/j.jmb.2011.04.076

Table 3.

Thermodynamic parameters obtained from titration of wild type (wt) BA2930 and two mutants H183G and H183A with CoA or AcCoA.

Protein	Ligand	K_D (µM)	ΔH (kcal·mol⁻¹)	−TΔS (kcal·mol⁻¹)	ΔG (kcal·mol⁻¹)	N
wt	AcCoA	33.35±2.53	−21.71±0.46	−15.61	−37.32	1.140±0.013
wt	CoA	6.23±1.81	−12.47±0.53	−5.36	−17.83	1.000±0.031
H183G	AcCoA	6.10±0.24	−14.83±0.10	−7.72	−22.55	0.750±0.004
H183G	CoA	10.27±1.53	−14.48±0.52	−7.66	−22.14	0.670±0.017
H183A	AcCoA	3.72±0.29	−17.40±0.20	−9.98	−27.39	0.610±0.005
H183A	CoA	8.69±0.69	−14.79±0.25	−7.87	−22.66	0.670±0.008