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. 2011 Jun 8;9(6):984–993. doi: 10.3390/md9060984

Figure 4.

Figure 4

Growth of the transformants of M. smegmatis over-expressing candidate area showing resistance to halicyclamine A on the 7H10 agar plate containing halicyclamine A. (a) Gene map of the cloning area to prepare transformants of M. smegmatis. (b) Each strain (wild-type of M. smegmatis, ICHO1007, ICHO1008, ICHO1009, ICHO1010, ICHO1011 and ICHO1012) was cultured in the 7H9 broth, and then the cultures were adjusted to 1 × 105 CFU/mL. The 10 μL aliquots of each culture were spread on the 7H10 agar plates in the presence or absence of halicyclamine A (5.0 μg/mL).