Table 1.
List of the transformants of M. smegmatis and description of plasmids.
| Strains | Plasmids | Description of plasmids |
Sequence of primers (5’ to 3’) F; Forward primer, R; Reverse primer |
|---|---|---|---|
| ICHO1007 | pMV206 | Cloned S1 area# (2919991–2923343 bp of M. bovis BCG genome)* | F:TCTAGAGCCACACCCTGATAGCATTG R: AAGCTTTCGCGACGGCTGGAATG |
| ICHO1008 | pMV206 | Cloned S2 area # (2924490–2923316 bp of M. bovis BCG genome)* | F:GGATCCAACTGTTTCCGCACGAGGAG R:CTGCAGTCAACGGCGAACATTCCAGC |
| ICHO1009 | pMV261 | Cloned S3 area # (2926966–2924589 bp of M. bovis BCG genome)* | F:GGATCCATCCAGGAGGTCGCAGATGTC R: GAATTCGTGATGTTCGCGCCTAACGG |
| ICHO1010 | pMV206 | Cloned S4 area # (2926766–2929363 bp of M. bovis BCG genome)* | F:CAGCTGATAGCTCTGCGCGTACGAC R: TTCGAAGATCAGCAACGGGCTGGAC |
| ICHO1011 | pMV206 | Cloned S5 area # (2929454–2931112 bp of M. bovis BCG genome)* | F: GGATCCTGCGGTATCGCTTGCCTTG R: AAGCTTGCGCGACTACGCAGCTC |
| ICHO1012 | pMV206 | Cloned S6 area # (2930898–2933532 bp of M. bovis BCG genome)* | F:GGATCCAAGCGGCTAACTGTAGGCCTG R: AAGCTTTCAGCCCGGTGGTGTTGG |
| ICHO1013 | pMV261 | Cloned BCG2662 gene (2926980–2927222 bp of M. bovis BCGgenome)* | F:GAATTCAGGACAACCCGCATGGTCG R: AAGCTTTATCACTCAGCGCCGGTACG |
| ICHO1014 | pMV261 | Cloned BCG2663 gene (2927223–2927900 bp of M. bovis BCG genome)* | F: GAATTCCCGGTCAGGCATTCTTTACCC R:AAGCTTGAAAGTTCCAGTTGCGCACGAC |
| ICHO1015 | pMV261 | Cloned BCG2664 gene(2928096–2928752 bp of M. bovis BCG genome)* | F: GAATTCCCGGTCAGGCATTCTTTACCC R: AAGCTTCAAGCCGGTCGGTCACG |
| ICHO1016 | pMV261 | Cloned BCG2665 gene(2928915–2929361 bp of M. bovis BCG genome)* | F: GGATCCATGCACCATGGGCCTGATCAC R: GAATTCTACGGCCGGGATCAGCAAC |
#;
Name of area corresponds to the Figure 4a.
*;
Coordinate of M. bovis BCG genome is based on the database of BCGList (http://genolist.pasteur.fr/BCGList/).