Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jun 7;12(6):3705–3722. doi: 10.3390/ijms12063705

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Optimum conditions for PEI max-MNPs magnetofection. To optimize conditions, we varied volume (A) and time on the magnetic plate (B). These results were evaluated by quantitative real-time RT-PCR. The relative expression level (GFP/GAPDH) in the human fetal lung-derived fibroblasts (TIG-1 cells) treated with PEI max alone (A), and in the absence of magnetic force (0 h) (B) was defined as 1. Optimal transfection conditions were established when TIG-1 cells were treated with 0.8 μg PEI max-MNPs and 2.0 μg pCAG-GFP for 8 h on the magnetic plate in either a six-well plate or a 35 mm dish. The asterisk (*) indicates a significant difference (P < 0.05).